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Thermo Fisher cm dii
Cm Dii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cm+dii/PHOSPHATE+BUFFERED+SALINE+PBS/pm41160998-463-24-25
Average 99 stars, based on 1 article reviews

cm dii - by Bioz Stars, 2026-09

99/100 stars

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Infection:

Article Title: Mosquito immune cells enhance dengue and Zika virus dissemination in Aedes aegypti
Article Snippet: .. At 7 days post-DENV or -ZIKV infection, mosquitoes were injected with 69 nl of a solution containing 100μM Vybrant CM-DiI (ThermoFisher Scientific) and 2% green fluorescent beads in 1X PBS, then incubated under insectary conditions for 30 min. To preserve hemocyte attachment, mosquitoes were injected with 207 nl of 50% ethanol in 1X PBS. ..

Injection:

Article Title: Identification of small molecules that suppress cell invasion and metastasis promoted by WASF3 activation
Article Snippet: Horseradish peroxidase-conjugated anti-rabbit was used as a secondary antibody (GE Healthcare NA934-1 ML). .. Metastasis assays were carried out essentially as described previously [ ] with fluorescent cell tracker CM-DiI (Invitrogen, Carlsbad, CA, USA) labeled human metastatic cells injected into the perivitelline space of 3-day-old embryos. ..

Article Title: Promotion of adipose stem cell transplantation using GelMA hydrogel reinforced by PLCL/ADM short nanofibers
Article Snippet: .. Rat ADSCs were fluorescently labeled with CM-DiI (C7000, Invitrogen) before injection. .. CM-DiI was diluted to 1 mg ml−1 in dimethyl sulfoxide (DMSO, Solarbio) and further to 1 μg ml−1 in D-PBS (Solarbio).

Incubation:

Article Title: Epicardial extracellular vesicles modulate gene expression following ischemia-reperfusion injury in heart-on-a-chip
Article Snippet: .. 150μl total of pooled NT- and FGF-EVs (100x concentrated) were mixed at a 1:3 ratio by volume with Cell Tracker CM-DiI (Invitrogen C7001; 1 mg/ml in DMSO, diluted to 5μg/ml with PBS) and incubated for 15 minutes in the dark at room temperature. .. Stained Epi-EVs were then added to an Amicon Ultra-0.5 ml 100kDa filter unit (MilliporeSigma UFC510024) and centrifuged for 5 min at 14 000 g. Two PBS washes of EVs were performed by adding 500μl PBS to the filter unit, mixing gently, then centrifuging for 5 min at 14 000 g. The filter unit was then flipped upside-down into a fresh collector tube and centrifuged for 2 min at 1000 g to collect washed and stained Epi-EVs.

Transferring:

Article Title: Tlx3 mediates neuronal differentiation and proper condensation of the developing trigeminal ganglion.
Article Snippet: The trigeminal ganglion, the largest of the vertebrate cranial ganglia, is comprised of sensory neurons that relay sensations of pain, touch, and temperature to the brain.. These neurons are derived from two embryonic cell types, the neural crest and ectodermal placodes, whose interactions are critical for proper ganglion formation.. While the T-cell leukemia homeobox 3 (Tlx3) gene is known to be expressed in placodally-derived sensory neurons and necessary for their differentiation, little was known about Tlx3 expression and/or function in the neural crest-derived component of the developing trigeminal ganglion.

Labeling:

Cell Culture:

Article Title: The multifaceted mechanism of action of quinoline thiosemicarbazone based on disruption of iron homeostasis and selective IGF1R inhibition leading to oxidative stress induction.
Article Snippet: .. Tumor LN-229 cell lines were cultured until cells reached 70 % of confluence, then washed with phosphate-buffered saline (PBS), detached, and stained with Vybrant CM-DiI (ThermoFisher; 4 μL/mL in 1X PBS) for 10 min at 37 ◦C in darkness. ..

Saline:

Staining:

Image Search Results

a Experimental design for ( b – e ). Newly emerged worker bees were divided into GF (germ-free), HK (inoculated with heat-killed gut homogenate), and CV (inoculated with nurse bee gut homogenate) groups. Gut bacterial inoculation was conducted within one day after emergence. Bees were maintained for 5 days prior to Hafnia injection, and separate groups of bees were used for the survival assay, gene expression analysis, and confocal microscopy. Created in BioRender. Liu, L. (2025) https://BioRender.com/en83bag . b Survival rates for GF, HK, and CV bees post Hafnia injection. Bars represent mean ± s.d. from three biological replicates (20 individuals in each replicate). The results are from one of two independent experiments (Supplementary Fig. for replicate data). A two-sided Mantel-Cox test was used. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). c Antimicrobial peptide (AMP) gene expression in fat bodies of GF, HK, and CV bees, measured by qPCR (SYBR Green, Actin -normalized). Bars represent mean ± s.e.m. n = 6 individuals for each group from 3 cup cages. d Ventral view of bee abdominal tergum showing the dorsal vessel. Yellow arrows indicate the ostia. e Hemocyte aggregation post-GFP- Hafnia injection (green). Hemocytes were stained with CM-DiI (red), and dorsal vessels were outlined by white dashed lines. Scale bars: 250 μm. Representative images from one of two independent experiments (Supplementary Fig. – for replicate data). f Quantitative analysis of CM-DiI staining in hemocytes (see Fig. 1e and Supplementary Fig. ). Bars represent mean ± s.e.m. n = 5 individuals per group from 3 cup cages. ( c , f ) Two - way ANOVA with Tukey’s multiple comparisons test. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). ns: not significant. All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming

doi: 10.1038/s41467-026-69073-0

Figure Lengend Snippet: a Experimental design for ( b – e ). Newly emerged worker bees were divided into GF (germ-free), HK (inoculated with heat-killed gut homogenate), and CV (inoculated with nurse bee gut homogenate) groups. Gut bacterial inoculation was conducted within one day after emergence. Bees were maintained for 5 days prior to Hafnia injection, and separate groups of bees were used for the survival assay, gene expression analysis, and confocal microscopy. Created in BioRender. Liu, L. (2025) https://BioRender.com/en83bag . b Survival rates for GF, HK, and CV bees post Hafnia injection. Bars represent mean ± s.d. from three biological replicates (20 individuals in each replicate). The results are from one of two independent experiments (Supplementary Fig. for replicate data). A two-sided Mantel-Cox test was used. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). c Antimicrobial peptide (AMP) gene expression in fat bodies of GF, HK, and CV bees, measured by qPCR (SYBR Green, Actin -normalized). Bars represent mean ± s.e.m. n = 6 individuals for each group from 3 cup cages. d Ventral view of bee abdominal tergum showing the dorsal vessel. Yellow arrows indicate the ostia. e Hemocyte aggregation post-GFP- Hafnia injection (green). Hemocytes were stained with CM-DiI (red), and dorsal vessels were outlined by white dashed lines. Scale bars: 250 μm. Representative images from one of two independent experiments (Supplementary Fig. – for replicate data). f Quantitative analysis of CM-DiI staining in hemocytes (see Fig. 1e and Supplementary Fig. ). Bars represent mean ± s.e.m. n = 5 individuals per group from 3 cup cages. ( c , f ) Two - way ANOVA with Tukey’s multiple comparisons test. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). ns: not significant. All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Article Snippet: At 3, 6, 12, and 24 h after Hafnia infection, bees were injected thoracically with 2 μL of a solution containing 67 μM Celltracker CM-DiI (Cat#40718ES50, Yeasen, China) and 1.08 mM Hoechst 33342 (Cat#C0031, Solarbio, China) in PBS.

Techniques: Injection, Clonogenic Cell Survival Assay, Gene Expression, Confocal Microscopy, SYBR Green Assay, Staining

a Survival rates of GF, CL (inoculated with a set of five core bacteria mixed at equal numbers), acetate-supplemented, and butyrate-supplemented bees following Hafnia injection. Bars represent mean ± s.d. n = 3 replicates for each group (30 individuals per replicate). The results are from one of two independent experiments (Supplementary Fig. for replicate data). A two-sided Mantel-Cox test was used. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). b Abdominal butyrate concentrations for GF, CL, and single bacterium-inoculated bees 5 days post-colonization. Ga: Gilliamella ; Sa: Snodgrassella ; Ba: Bifidobacterium ; F4: Bombilactobacillus ; F5: Lactobacillus . The isolates for each strain are listed in Supplementary Table . Bars represent mean ± s.e.m. n = 3 replicates for each group, each replicate pooled from 3 individuals from 3 cup cages. The experiment was repeated twice with similar results. One-way ANOVA with the Tukey post hoc test. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). c AMP expression in the fat bodies of GF, CV, CL, and butyrate-supplemented bees, before Hafnia infection and 3 h after infection (GF as control). Bars represent mean ± s.e.m. n = 8 individuals for each group from 3 cup cages. Gene expression was quantified using qPCR as shown in Fig. . Two-sided Mann–Whitney test and two-sided unpaired Student’s t test, depending on data normality. ns: not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001. Created in BioRender. Liu, L. (2025) https://BioRender.com/hdvmsxq . d CM-DiI-stained hemocytes (red) of GF, HK, CL, and butyrate-supplemented bees before infection and at 3 h post-GFP- Hafnia infection (green). Dorsal vessels were outlined with white dashed lines. Scale bars: 250 μm. Representative images from one of two independent experiments (see Supplementary Figs. , for replicate data). All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming

doi: 10.1038/s41467-026-69073-0

Figure Lengend Snippet: a Survival rates of GF, CL (inoculated with a set of five core bacteria mixed at equal numbers), acetate-supplemented, and butyrate-supplemented bees following Hafnia injection. Bars represent mean ± s.d. n = 3 replicates for each group (30 individuals per replicate). The results are from one of two independent experiments (Supplementary Fig. for replicate data). A two-sided Mantel-Cox test was used. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). b Abdominal butyrate concentrations for GF, CL, and single bacterium-inoculated bees 5 days post-colonization. Ga: Gilliamella ; Sa: Snodgrassella ; Ba: Bifidobacterium ; F4: Bombilactobacillus ; F5: Lactobacillus . The isolates for each strain are listed in Supplementary Table . Bars represent mean ± s.e.m. n = 3 replicates for each group, each replicate pooled from 3 individuals from 3 cup cages. The experiment was repeated twice with similar results. One-way ANOVA with the Tukey post hoc test. Different letters above bars indicate statistically significant differences among treatments ( P < 0.05). c AMP expression in the fat bodies of GF, CV, CL, and butyrate-supplemented bees, before Hafnia infection and 3 h after infection (GF as control). Bars represent mean ± s.e.m. n = 8 individuals for each group from 3 cup cages. Gene expression was quantified using qPCR as shown in Fig. . Two-sided Mann–Whitney test and two-sided unpaired Student’s t test, depending on data normality. ns: not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001. Created in BioRender. Liu, L. (2025) https://BioRender.com/hdvmsxq . d CM-DiI-stained hemocytes (red) of GF, HK, CL, and butyrate-supplemented bees before infection and at 3 h post-GFP- Hafnia infection (green). Dorsal vessels were outlined with white dashed lines. Scale bars: 250 μm. Representative images from one of two independent experiments (see Supplementary Figs. , for replicate data). All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Techniques: Bacteria, Injection, Expressing, Infection, Control, Gene Expression, MANN-WHITNEY, Staining

a PCA of abdominal metabolomes from GF and butyrate-supplemented bees at day 5 post-supplementation (n = 6 per group, each point represents one biological replicate). b Heatmap of the discriminating metabolites in the arachidonic acid pathway in the abdomen of GF and butyrate-supplemented bees. c Prostaglandin E 2 (PGE 2 ) levels in abdomen, hindgut and hemolymph in GF, CV, CL, and butyrate-supplemented bees at day 5 post-colonization (abdomen: n = 6; hindgut and hemolymph: n = 4; each pooled from 5 individuals from 3 cup cages). One of two independent experiments (Supplementary Fig. ). Two-sided Mann–Whitney and two-sided unpaired Student’s t test, depending on data normality. d Knockdown of fat body Pla2 in GF bees following dsRNA injection (GFP dsRNA as control). n = 8 per group from 3 cup cages. P = 0.0394, two-sided Welch’s t test. e Effect of Pla2 RNAi on PGE 2 levels in fat bodies of GF and butyrate-supplemented bees ( n = 5 replicates per group, each pooled from 5 individuals from 3 cup cages). P = 0.0039, two-sided Student’s t test. f Survival of GF, GF + PGE 2 , CL, and CL+acetylsalicylic acid (ASA) bees post- Hafnia infection. Bars represent mean ± s.d. from three replicates per group (30 individuals per replicate) from one of two independent experiments (Supplementary Fig. ). Two-sided Mantel–Cox test; different letters indicate significant differences ( P < 0.05). g AMP expression in fat bodies before and 3 h post- Hafnia infection (GF as control; n = 8 per group from 3 cup cages. Two-sided Mann–Whitney and two-sided unpaired Student’s t test, depending on data normality. h In vivo hemocyte staining using CM-DiI (red) before infection (3 h after PGE 2 supplementation) and at 3 h post-infection with GFP- Hafnia (green). Dorsal vessels were outlined with white dotted lines. Scale bars: 250 μm. Representative images from two independent experiments (Supplementary Figs. , ). In ( c – e , g ), bars represent mean ± s.e.m. * P < 0.05, ** P < 0.01, and *** P < 0.001. All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming

doi: 10.1038/s41467-026-69073-0

Figure Lengend Snippet: a PCA of abdominal metabolomes from GF and butyrate-supplemented bees at day 5 post-supplementation (n = 6 per group, each point represents one biological replicate). b Heatmap of the discriminating metabolites in the arachidonic acid pathway in the abdomen of GF and butyrate-supplemented bees. c Prostaglandin E 2 (PGE 2 ) levels in abdomen, hindgut and hemolymph in GF, CV, CL, and butyrate-supplemented bees at day 5 post-colonization (abdomen: n = 6; hindgut and hemolymph: n = 4; each pooled from 5 individuals from 3 cup cages). One of two independent experiments (Supplementary Fig. ). Two-sided Mann–Whitney and two-sided unpaired Student’s t test, depending on data normality. d Knockdown of fat body Pla2 in GF bees following dsRNA injection (GFP dsRNA as control). n = 8 per group from 3 cup cages. P = 0.0394, two-sided Welch’s t test. e Effect of Pla2 RNAi on PGE 2 levels in fat bodies of GF and butyrate-supplemented bees ( n = 5 replicates per group, each pooled from 5 individuals from 3 cup cages). P = 0.0039, two-sided Student’s t test. f Survival of GF, GF + PGE 2 , CL, and CL+acetylsalicylic acid (ASA) bees post- Hafnia infection. Bars represent mean ± s.d. from three replicates per group (30 individuals per replicate) from one of two independent experiments (Supplementary Fig. ). Two-sided Mantel–Cox test; different letters indicate significant differences ( P < 0.05). g AMP expression in fat bodies before and 3 h post- Hafnia infection (GF as control; n = 8 per group from 3 cup cages. Two-sided Mann–Whitney and two-sided unpaired Student’s t test, depending on data normality. h In vivo hemocyte staining using CM-DiI (red) before infection (3 h after PGE 2 supplementation) and at 3 h post-infection with GFP- Hafnia (green). Dorsal vessels were outlined with white dotted lines. Scale bars: 250 μm. Representative images from two independent experiments (Supplementary Figs. , ). In ( c – e , g ), bars represent mean ± s.e.m. * P < 0.05, ** P < 0.01, and *** P < 0.001. All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Techniques: MANN-WHITNEY, Knockdown, Injection, Control, Infection, Expressing, In Vivo, Staining

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cm dii (Thermo Fisher)

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